Extended Data Fig. 9: Chemostat cultivations of C. necator ΔphaC1 and CRG6 ΔdadA6 on formate.
From: One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle
Strains were cultivated in a carbon limited chemostat at a dilution rate of 0.05 h−1 with a 80 mM formic acid feed in 10 % CO2 (at 2.5 vvm). Process data from the entire ΔphaC1 and CRG6 ΔdadA6 cultivations are shown. Infrared based cell density data and pH values are depicted. The 3 day harvest window during which samples were taken for cell dry weight analysis is indicated in grey. The dilution rate was set to 0.087 h−1 equal to 8 h doubling time after 1000 h of cultivation and to 0.1 h−1 after ~1300 h of cultivation, after which the cells washed out.
