Fig. 2: Characterization of the rGlyP and CBB cycle C. necator strains.
From: One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle
a, Growth of C. necator H16 ΔphaC1 strains harbouring the CBB cycle compared with that of CRG4, CRG5 and CRG6 in M9 minimal media supplemented with 80 mM formate and 100 mM bicarbonate with 10% CO2 in the headspace. The doubling time in hours of the strains is presented in the designated strain colour. Curves depict the mean of at least two technical replicates and are representative of three experiments conducted in the same conditions to ensure reproducibility. b, Relative protein intensity changes of the rGlyP modules in strain CRG6 relative to CRG4 both grown on formate minimal media. c, Fractions of the quantified proteome associated with various metabolic tasks in the strains CBB, CRG4 and CRG6 all grown on formate minimal media. Clustering criteria for grouping of proteins by metabolic tasks are provided in Methods. d, Measured biomass yields in grams CDW per mole formate consumed are shown, both for the CBB cycle (C. necator ΔphaC1) and rGlyP (CRG6 ΔdadA6) strains grown in formate minimal media in bioreactors in chemostat mode at a dilution rate of 0.05 h−1 (14 h doubling time). Predicted biomass yields are shown in a shaded pattern and are derived from FBA simulations run with a fixed growth rate corresponding to a doubling time of 14 h and maintenance costs of GAM = 135 mmol gCDW−1 and NGAM = 3 mmol gCDW−1 h−1. Measured data represent samples (n = 9) obtained during steady state, each having a volume of 50 ml or 100 ml taken over the course of 3 days. All data points and their mean value are given. Error bars indicate standard deviation. Significance was tested via two-tailed unpaired t-test. ****P = 1.33 × 10−6; 95% confidence interval = 0.4600–0.8244; difference between means ± s.e.m. = 0.6422 ± 0.08594; d.f. = 8.
