Extended Data Fig. 1: The genetic integration workflow employed in this study.
From: One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle
The two synthetic operons previously expressed from plasmids were genomically integrated via random Tn5-transposon integration with a library of different strength promoters in two consecutive rounds. The generated genetic libraries were grown on formate medium in 10 rounds to select for the fastest growing strains.
