Extended Data Fig. 3: Creation of CRG5 and CRG6 strains.
From: One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle
Liquid selection test tube experiment on M9, 80 mM formate, 100 mM HCO3 minimal media in 10% CO2 for creation of the CRG5 gP14-C1 (a) and CRG6 gP3-C3 strains (b). Following transposon integration of module 1 or module 3 of the rGlyP and upon reaching early stationary phase cells were re-inoculated into fresh formate media. Dots represent the measured glass tube OD600 values of two biological replicates per promoter operon transposon insertion. The lines correspond to their averages. 4 passages are depicted and are indicative of the improved growth over the 10 passages.
