Extended Data Fig. 2: Cycling genes in mosquitos.
From: Parasite and vector circadian clocks mediate efficient malaria transmission
A. Histograms showing that across 1,000 permutations of Anopheles stephensi genes in both light/darkness and complete darkness over 72 h, no permutation had more cycling genes than the observed sample, as determined by a combination of cycle detection algorithms. This analysis shows that an average of 70-120 genes would be defined as cycling by chance, whereas the correct time point order identified between 3000 to 5000 genes. B. Confirmation of salivary gland transcriptome. Normalized expression of 443 fat-body-specific genes were obtained from VectorBase (Sreenivasamurthy, Madugundu et al. 2017), where the top 100 expressed genes were plotted (pink violin plot) and compared their expression levels to our top 100 expressed fat-body-specific genes in our salivary gland transcriptome dataset in complete darkness (black horizontal line). Unpaired Welch’s t test, two-sided, ****p-value < 0.0001. C. Phase of known clock genes of insects. D. Expression profile of known clock genes of mosquitos insects. E. Top enriched cycling genes shared INTERPRO domains across conditions. F. Top enriched cycling genes shared KEGG pathways across conditions. For E/F: one-sided; used Benjamini-Hochberg (FDR) method to correct for multiple comparisons.
