Extended Data Fig. 3: Proteomics of infected salivary glands across four times of the day.
From: Parasite and vector circadian clocks mediate efficient malaria transmission
A. Number of proteins detected in infected A. stephensi salivary gland samples, across 3 replicates at ZT0 (Zeitgeber 0), ZT6, ZT12, and ZT18. 1,480 proteins were found to be common across all timepoints and replicates (left-most bar). Remaining bars on the plot represent the groups of proteins shared across a portion of samples but not detected across all time points and replicates. B. Boxplot of log2 protein intensities (protein expression) for all proteins across each of the four timepoints and its three replicates, colored by timepoint. Each replicate (each box plot of the same color) for each timepoint shows log2 intensities from all identified proteins from lysate of 5 infected salivary glands. Middle line on the boxplot indicates mean log2 intensity for all proteins detected within that replicate. For boxplot features, please refer to source data provided. C. Volcano plots of proteins identified from infected salivary glands (gray points) and whether they were differentially expressed (red points). Top-left panel: Proteins found to be differentially expressed (DE) at ZT12 vs ZT18. Top-middle panel: Proteins found to be DE at ZT12 vs ZT6. Top-right panel: Proteins found to be DE at ZT18 vs ZT6. Bottom-left panel: Proteins found to be DE at ZT0 vs ZT12. Bottom-middle panel: Proteins found to be DE at ZT0 vs ZT18. Bottom-right panel: Proteins found to be DE at ZT0 vs ZT6. Table S6 contains significance testing and fold-change calculation using MSstats. Red dotted line indicates cutoffs for determining DE using p-values and log2 intensities of fold change (thresholds of p-value <= 0.05, FC > = 2). Statistical analyses were performed using unpaired t-test, two-sided. Red points show DE proteins between two designated timepoints for each plot.
