Extended Data Fig. 4: Cycling genes and proteins in mosquito salivary glands and Plasmodium sporozoites.
From: Parasite and vector circadian clocks mediate efficient malaria transmission
A. Salivary gland protein expression and transcript expression profiles across time for some DE proteins. Left plots: Mean log2 Intensities (red line) of proteins DE across time. Each blue point represents log2 intensity value for one of three replicates. Right plots: For all proteins plotted, their transcripts cycle throughout the day. Bottom-most protein and transcript is a blood-meal related gene. B. Histograms showing that across 1,000 permutations of Plasmodium genes in both light/darkness and complete darkness over 72 h, no permutations had more cycling genes than the observed sample (green dot), as determined by a combination of cycle detection algorithms. This analysis showed that on average, only 22 genes would be recognized as cycling by chance, whereas the real time point order identified between 500 to 950 cycling genes.
