Extended Data Fig. 4: Induction of Th1, Th2 and Th17 cytokines by MLN and splenic CD4⁺ T cells from preweaning mice given L. animalis^WU with and without EGF.

(a) Flow cytometry gating strategy for identifying Th1 cytokines (TNFα and IFNɣ) secreted by CD45⁺CD3⁺CD4⁺ T cells in MLNs and spleens. Frequency of TNFα+ and IFNɣ + CD4 + T cells in (b-c) MLNs and (d-e) spleens of nontreated (control), L. animalis^WU fed and L. animalis^WU + EGF fed mice. (f) Flow cytometry gating strategy for identifying Th2 cytokines (IL4 and IL13) secreted by CD45⁺CD3⁺CD4⁺ T cells in MLNs and spleens. Frequency of IL13+ and IL4 + CD4 + T cells in (g-h) MLNs and (i-j) spleens of nontreated (control), L. animalis^WU fed and L. animalis^WU + EGF fed mice. (k) Flow cytometry gating strategy for identifying Th17 cytokines (IL17 and IL22) secreted by CD45⁺CD3⁺CD4⁺ T cells in MLNs and spleens. Frequency of IL17+ and IL22 + CD4 + T cells in (l-m) MLNs and (n-o) spleens of nontreated (control), L. animalis^WU fed and L. animalis^WU + EGF fed mice. Statistical analyses were performed by one-way ANOVA with Dunnett’s post test. Graphs represent mean + /− SEM. P values are as denoted.