Extended Data Fig. 9: 3-methyl-NQNO has potent and selective antibiotic activity against N. gonorrhoeae.

(a) Growth of N. gonorrhoeae MS11 and N. cinerea in the presence of the indicated concentrations of 3Me-NQNO (left: high concentration range 50–5 µM; right: low concentration range: 2.5 – 0.1 µM). Growth was determined by OD₅₅₀ readings every 30 min over 8 h. Shown is a representative experiment repeated twice with similar results. (b) The indicated Lactobacillus species were grown in the presence of 5, 10, 25, or 50 µM 3Me-NQNO. Control cultures received solvent (1% DMSO) only. Growth was monitored by OD₆₀₀ readings every 30 min. Growth was also quantified by the area under the growth curve. The color gradient used indicates growth of compound-treated cultures compared to 1% DMSO control and ranges from no growth (90–100 % inhibition - white) to full growth (0–10 % inhibition – dark green).