Extended Data Fig. 5: Gating strategy for flow cytometry analysis and cell sorting. | Nature Microbiology

Extended Data Fig. 5: Gating strategy for flow cytometry analysis and cell sorting.

From: Gut commensal Phascolarctobacterium faecium retunes innate immunity to mitigate obesity and metabolic disease in mice

Extended Data Fig. 5

(a) Bone marrow-derived macrophages (BMDMØ) were characterized as CD11c+CD11bhighMHC-II + CD115+ cells and polarization to M2 was determined by the expression of Arg-1, CD206 and/or CD163. (b) Sorted Innate lymphoid cells group 1 (ILC1) isolated from the small intestine were defined as CD45.2+Lin-CD127 + CD90.2 + NK1.1 + NKp46+ cells. The lineage cocktail included: CD3ε, CD8a, CD19, Ter119, Cd11c, TCRb, TCRgd, Gr1, Cd11b.

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