Extended Data Fig. 5: Imaging the fate of ICP1 infected cells.
From: West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems
(a) Time-course microscopy snapshots comparing the growth and cell morphology of V. cholerae A1552 cultures at 1, 2, 3, 4 and 5 hours after infection with ICP1-2006 at MOI 5, as compared to an uninfected control culture (+ LB). Note how the ICP1-infected culture initially contains only a few cells with normal morphology (white arrowheads), and that these cells increase in number as the culture rebounds, while the swollen ICP1-infected cells that are initially dominant persist for several hours before undergoing lysis (yellow arrowheads). (b) SytoxBlue-staining of V. cholerae A1552 cultures 1 hour after infection with ICP1-2006 at MOI 5, as compared to an uninfected control culture (+ LB). (c) Plaque assays testing the ability of ICP1-2006 and ICP3-2007 (which both require the O1 antigen receptor) to form plaques on cultures of A1552ΔWASA-1 that rebounded following ICP1-infection, as compared to a culture of the same strain that was grown without selection, and the set of indicated control strains. Note how neither ICP1-2006 nor ICP3-2007 are able to form plaques on the rebounded cultures, consistent with recovery of the culture being driven by the growth of spontaneous O1-antigen mutant cells. All images are representative of the results of three independent experiments. Scale bars = 20 µm.
