Extended Data Fig. 7: Anti-phage activity of GrwAB and VcSduA in E. coli.
From: West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems
(a) Fold-protection against E. coli phages of the BASEL collection conferred by the production of either GrwA, GrwB, VcSduA, GrwAB or GrwAB-VcSduA in E. coli MG1655ΔaraCBAD, as compared to a negative ‘no system’ control. Genes were expressed from a chromosomally integrated transposon carrying the arabinose-inducible PBAD- promoter, induced by the addition of 0.2% arabinose. Bar charts show the mean of two independent experiments. Bars with a black dot indicate phages exhibiting altered plaque morphology. (b) Representative examples of the altered plaque morphology phenotype observed for Bas03 and Bas05 in the presence of VcSduA production.
