Extended Data Fig. 8: Identification of conserved motifs and distribution of GrwAB.
From: West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems
(a, d) Sequence logos showing conservation of the identified QR, DGQQR and FxxxN motifs in the DUF262 domains of the 471 GrwA (a) and 471 GrwB (d) hits detected using MacSyFinder v.2.1.1, as compared to the equivalent residue number in V. cholerae A1552 GrwA and GrwB. Amino acids in logos are coloured according to chemical properties: polar (G, S, T, Y, C), green; neutral (Q, N), purple; basic (K, R, H), blue; acidic (D, E), red; and hydrophobic (A, V, L, I, P, W, F, M), black. (b) Sequence logo showing the conservation of the identified His-Me (HNH) nuclease motif in the DUF1524 domain of the 471 GrwA hits detected using MacSyFinder v.2.1.1, as compared to the equivalent residue number in V. cholerae A1552 GrwA. (c) Location of the identified His-Me (HNH) nuclease motif residues in the GrwA structural prediction, highlighting the predicted ββα fold. (e) AlphaFold3 predicted structures of potential GrwAB complexes. Colour scheme as in (c). (f) Distribution of GrwAB hits detected using MacSyFinder v.2.1.1. The tree shows the order-level phylogeny of genera in the RefSeq database with more than 500 genomes (see methods). For the full list of 471 GrwAB hits see Supplementary Table 6.
