Extended Data Fig. 9: Bas24 escaper phages overcome GrwAB defence via mutations in the DNA modification pathway.
From: West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems
(a) Plaque assays evaluating the ability of 10-fold serial dilutions of Bas24 escaper phages to form plaques on E. coli MG1655ΔaraCBAD in the absence (No system) and presence of GrwAB (+ GrwAB), as compared to the parental Bas24 stock and a control stock propagated in the absence of GrwAB. (b) SNP table summarising results of whole genome sequencing for each phage compared to the published Bas24 reference sequence. Light yellow (SNP), dark yellow (SNP targeting dpdA locus), HP (hypothetical protein). (c) Multiple-sequence alignment showing conservation of Queuovirinae (Bas19-25) homologues of the 7-deazaguanosine inserting enzyme DpdA, with the residues found to be substituted in the Bas24 escaper phages highlighted in red. (d) Locations of the substituted residues shown on predicted structure of Bas24 DpdA, generated using AlphaFold3, compared to the experimentally determined crystal structure of Salmonella enterica serovar Montevideo DpdA (PDB 7UI4) showing the active site highlighted in magenta.
