Fig. 2: WonAB protects cells by lysis-independent abortive infection.
From: West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems
a, Growth kinetics of V. cholerae strain A1552 in the presence (WT) and absence of WASA-1 (ΔWASA-1), with either no phage or infected at time 0 with ICP1-2006 at the indicated MOI. Data are representative of three independent experiments. b, Time-lapse microscopy comparing exponentially growing cells of V. cholerae A1552 WT and ΔWASA-1 strains after infection with ICP1-2006 at MOI 5. c, Fold change in ICP1-2006 genome copy number relative to time 0, as determined by qPCR, after infection of either WT or ΔWASA-1 strains with ICP1-2006 at MOI 0.1. The chart shows the mean ± s.d. of three independent experiments. d, Time-course microscopy snapshots comparing cell morphology and cellular DNA content, as monitored using HU–mNeonGreen fusion (mNG), in WT and ΔWASA-1 backgrounds, following infection with ICP1-2006 at MOI 5. e–g, Time-course microscopy snapshots comparing ICP1 capsid assembly, as monitored by the incorporation of a host-cell-produced mNG fusion to the ICP1 major capsid protein Gp122 (e), in either WT (f) or ΔWASA-1 (g) backgrounds, following infection with ICP1-2006 at MOI 5. All images are representative of the results of three independent experiments. Scale bars, 5 µm.
