Fig. 5: VcSduA protects V. cholerae against vibriophage X29.
From: West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems
a, Plaque assay showing X29 PFU ml−1 on the pre-7th pandemic strain MAK757, in the presence and absence (ΔmanCB) of the O1-antigen. b, Tenfold serial dilution plaque assays of X29 on wave 1 V. cholerae strains, comparing the effect of deleting ddmABC in non-WASA (N16961, P27459, E7946) and WASA lineage (A1552, C6706) strains, below a pre-7th pandemic control (MAK757). c, Plaque assay showing X29 PFU ml−1 obtained on C6706 derivates deleted for combinations of ddmABC with ΔLAT1 (ΔgrwAB-VcSduA), ΔgrwAB and ΔVcSduA, alongside representative images of each tenfold serial dilution plaque assay. Note that strain C6706 was used in these assays because the rifampicin antibiotic resistance marker in strain A1552 partially interferes with X29 infection. d,e, Plaque assays testing the ability of VcSduA production to complement X29 protection in a C6706ΔddmABCΔVcSduA background (d) and in ΔddmABC backgrounds of the non-WASA linage strains E7946 and P27459 (e). The bar charts show the mean + s.d. of three independent experiments. The images are representative of the results of three independent experiments.
