Extended Data Fig. 3: Validation of those protein chains modelled in the scaffold map to subnanometre resolution.

(a) Map-model FSC curves for protein models refined into the scaffold map: FlgP, PflA, PflC1, and PflC2–7 calculated in Phenix83, alongside images of excised pieces of the density map corresponding to individual proteins. High and low isosurface thresholds are denoted by solid and mesh surfaces, respectively, to illustrate fit of models into secondary structure densities. (b) Cross-correlation per residue plots of proteins docked into the map: PflB, FliL, MotB, PflD, calculated using Phenix. Mean CC values are shown with dashed line and in text inset.