Fig. 1: Expression timings and localizations of P. falciparum FIKK kinases.
From: The fast-evolving FIKK kinase family of Plasmodium falciparum can be inhibited by a single compound
a, A heat map built using data from Hoeijmakers et al.74 RNA-sequencing dataset available on PlasmoDB (www.PlasmoDB.org), showing the percentage expression for each FIKK during the P. falciparum asexual replication cycle. Yellow, maximum expression; dark blue, minimum expression. The TPM values used to calculate the percentage expression relative to maximum expression across the 48 h lifecycle are available in Supplementary Table 2. b, A diagram illustrating P. falciparum FIKKs expression and localizations in iRBCs. Top left: an illustration of the P. falciparum lifecycle. FIKK1, FIKK4.1, FIKK8, FIKK9.3, FIKK12 and FIKK13 expressed in gametocytes are shown in orange. Bottom right: P. falciparum iRBC showing the localization of the FIKKs. Blue, FIKK3, FIKK5, FIKK8, FIKK9.2 and FIKK9.5 in the parasite; green, FIKK1, FIKK7.1, FIKK9.1, FIKK9.3, FIKK10.1, FIKK10.2 and FIKK12 in Maurer’s clefts; red, FIKK1, FIKK4.1, FIKK4.2 and FIKK12 at the RBC periphery. *Localization data from publications from other laboratories. Top right: knob structure at the RBC periphery. Yellow stars show FIKK4.1 substrates and orange stars show FIKK4.2 substrates (data from ref. 16). EDVs, electron dense vesicles. c, Western blots confirming expression of HA-tagged P. falciparum FIKKs in gametocytes stage III. GAP50 antibody (bottom) demonstrates equal loading. The arrows show FIKK bands at expected sizes (shown in the labels at the top). +Rapamycin treatment.
