Extended Data Fig. 6: KSHV vBcl-2 attenuates MAVS-mediated IFN response.
a-c, RT-qPCR analysis of IFNB1 (a) and ISG15 (c) mRNA levels (normalized to 18S rRNA), and IFN-β production (b) in supernatant measured by ELISA in iSLK-BAC16.vBcl-2 WT and vBcl-2 KO cells induced with Dox/NaB for the indicated time. n = 3. d-f, RT-qPCR analysis of vBcl-2 transcripts (d, n = 3), IFNB1 transcripts (e, n = 3), and ISG15 transcripts (f, n = 3) in TREx BCBL-1-RTA cells transfected with two independent vBcl-2-specific siRNA for 12 h and induced with Dox (1 μg/ml) and Nab (1 mM) for 48 h. g, RT-qPCR of IFNB1 mRNA in HCT116 cells infected with vBcl-2 WT or vBcl-2 KO KSHV at 10 MOI for 60 h (n = 3). h, Representative STED micrographs of MAVS (magenta) and TOM20 (green) in iSLK-BAC16.vBcl-2 WT cells transduced with Ctrl vs. DRP1 shRNAs or NM23-H2 shRNAs at 60 h post-reactivation. Insets highlight MAVS distribution. Arrows indicate MAVS puncta and dotted lines denote clustering. Data are from one experiment that is representative of three independent experiments. i, Diameter of MAVS clusters measured from images in h (n = 100). j. RT-qPCR of ISG15 mRNA expression in h (n = 3). Data in a-g represent mean ± s.d. obtained from three independent experiments performed in triplicates, and analyzed by two-tailed t test (a-c) or one-way ANOVA followed by Tukey’s post hoc test (d-g). Box-plot data in i are presented as median (center line), interquartile range (IQR; box, 25th to 75th percentiles), and whiskers extending from minimum to maximum values; and analyzed using Kruskal-Wallis with post hoc Dunn’s test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant. Exact P values are provided in Source Data Extended Data Fig. 6.
