Extended Data Fig. 5: Morphological data supports intra-cellular accumulation of PFAS.
From: Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances
a-f. Bacteria show distinct morphological features in transmission electron microscopy (TEM). a,b. Automatic identification of bacterial cells (a) and condensates (b) for B. uniformis cells grown in mGAM + 250 µM (116 mg/l) PFNA. 119 images analysed in total including all strains and exposures. c. Mean pixel intensity of bacterial cells show significant differences between DMSO and PFNA treated cells for B. uniformis, E. coli BW25113 and O. splanchnicus; two-sided t-test; p-values FDR corrected; Boxplot: centre = 50th percentile, bounds of box = 25th and 75th percentile, lower/upper whisker = lower/upper hinge −/+ 1.5 * inter quartile range; Number of cells analysed: B. uniformis: DMSO = 141, PFNA_250µM = 178; E. coli (BW25113): DMSO = 115, PFNA_250µM = 61; E. coli (BW25113) ∆tolC: DMSO = 56, PFNA_250µM = 14; O. splanchnicus: DMSO = 76, PFNA_250µM = 57 (Supplementary Table 37). d. Condensate count per bacterial cell show significant increase in condensates for B. uniformis and O. splanchnicus, supporting morphological changes through PFNA exposure; two-sided t-test; p-values FDR corrected; Number of cells analysed: B. uniformis: DMSO = 141, PFNA_250µM = 178; E. coli (BW25113): DMSO = 115, PFNA_250µM = 61; E. coli (BW25113) ∆tolC: DMSO = 56, PFNA_250µM = 14; O. splanchnicus: DMSO = 76, PFNA_250µM = 57 (Supplementary Table 37). e. B. uniformis data from c including data for 5, 50, 125 µM PFNA and 125 µM PFDA. Mean intensity of bacterial cells show significant differences between DMSO and PFNA or PFDA treated cells; two-sided t-test; p-values FDR corrected; Boxplot: centre = 50th percentile, bounds of box = 25th and 75th percentile, lower/upper whisker = lower/upper hinge −/+ 1.5 * inter quartile range; Number of cells analysed: DMSO = 141, PFNA_5µM = 34, PFNA_50µM = 29, PFNA_125µM = 26, PFNA_250µM = 178, PFDA_125µM = 38 (Supplementary Table 37. f. B. uniformis data from d including data for 5, 50, 125 µM PFNA and 125 µM PFDA. Condensate count per bacterial cell show significant increase in condensates for B. uniformis exposed to 50, 125 and 250 µM PFNA, supporting morphological changes through PFNA exposure; two-sided t-test; p-values FDR corrected; Number of cells analysed: DMSO = 141, PFNA_5µM = 34, PFNA_50µM = 29, PFNA_125µM = 26, PFNA_250µM = 178, PFDA_125µM = 38 (Supplementary Table 37).
