Extended Data Fig. 7: PFAS tolerance and bioaccumulation following adaptive laboratory evolution.
From: Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances
a. Five gut bacterial species were evolved through serial passaging in growth medium containing one of four PFAS compounds (500 μM PFHpA, 500 μM PFOA, 250 μM PFNA, 125 μM PFDA) over 20 days. b. Improved growth of adapted P. merdae in presence of 125 μM PFDA at days 5, 10, 15 and 20 compared to day 0 (day 20: 1.3-fold change, p-value = 0.001). n = 4 independent populations per compound (Supplementary Tables 38,39). c. Improved growth of adapted B. uniformis population in presence of 250 μM PFNA and 125 μM PFDA after 5, 10, 15 and 20 days compared to day 0 (day20 PFNA 7-fold change, p-value = 0.0004; day 20 PFDA 46-fold change, p-value = 0.00003). n = 4 independent populations per compound (Supplementary Tables 38,39). d. Improved growth of adapted E. coli BW25113 ΔTolC population in presence of 500 µM PFHpA, 500 µM PFOA, 250 μM PFNA and 125 μM PFDA at day 20 compared to day 0 (PFHpA 1.3-fold change, p-value = 0.002; PFOA 1.7-fold change, p-value = 0.0006; PFNA 2.3-fold change, p-value = 0.0006; PFDA 1.6-fold change, p-value = 0.00005). n = 4 independent populations per compound (Supplementary Tables 38,39). e. Adapted populations retain PFDA and PFNA bioaccumulation capability. Mean of n = 4 independent populations per compound (Supplementary Table 40).
