Extended Data Fig. 1: Method setup and results from the community-screen.
From: Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances
a. Method workflow for the artificial community experiment. Each community consisted of 10 bacteria (community 1: Bacteroides caccae, Bacteroides dorei, Bacteroides thetaiomicron, Bacteroides uniformis, Bacteroides vulgatus, Colinsella aerofaciens, Coprococcus comes, Eubacterium rectale, Parabacteroides merdae, Roseburia intestinalis; community 2: Akkermansia muciniphila, Bacteroides clarus, Bacteroides stercoris, Clostridium difficile, Eggerthella lenta, Eubacterium eligens, Fusobacterium nucleatum subsp. animalis, Odoribacter splanchnicus, Parabacteroides distastonis, Ruminococcus bromii). b. 13 out of 42 tested pollutants were sequestered by at least one synthetic gut bacterial community. Coloured squares denote sequestration, that is, >20% reduction from the bacteria-free supernatant. PFAS are marked in blue. n = 6 (3 biological and 2 technical replicates) (Supplementary Table 31). c. Method workflow for the single strain experiment.
