Extended Data Fig. 4: Transmission electron microscopy displays morphological changes upon PFAS exposure.
From: Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances
a-f. TEM of B. uniformis cells grown in mGAM + DMSO (59 images from 3 biological replicate) (a), 5 μM PFNA (31 images from 1 biological replicate) (b), 50 μM PFNA (40 images from 1 biological replicate) (c), 125 μM PFNA (51 images from 1 biological replicate) (d), 250 μM PFNA (79 images from 3 biological replicate) (e), or 125 μM PFDA (46 images from 1 biological replicate) (f) for 24 h. g,h. TEM of E. coli. BW25113 wild-type cells grown in mGAM + DMSO (40 images from 1 biological replicate) (g) or 250 μM PFNA (45 images from 1 biological replicate) (h) for 24 h. i,j. TEM of E. coli. BW25113 ∆tolC cells grown in mGAM + DMSO (31 images from 1 biological replicate) (i) or 250 μM PFNA (50 images from 1 biological replicate) (j) for 24 h. k,l. TEM of O. splanchnicus cells grown in mGAM + DMSO (37 images from 1 biological replicate) (k) or 250 μM PFNA (38 images from 1 biological replicate) (l) for 24 h. A tomogram for B. uniformis cells exposed to 250 µM PFNA confirming that the structures are inside the cells is available as a supplementary video.
