Extended Data Fig. 4: Molecular characterization of IL-10 producing CD4+ T cells in the small intestine lamina propria.
(a) UMAP plot showing the clustering analysis of the merged dataset of all sorted IL-10 producing CD4+ T subsets in the small intestine lamina propria from both naïve and Giardia-infected IL-10-GFP reporter mice. The plot distinctly identifies five key clusters, corresponding to Th1, Th2, Th17, Treg, and Th2 Treg cells subset. (b) Dot plot graph highlighting the top10 highly expressed genes within each cluster of IL-10 producing CD4+ T cells in the small intestine lamina propria from naïve and Giardia-infected mice. (c) UMAP plot of Giardia-induced IL-10-producing CD4+ T cells five clusters mapped against to public available scRNA-seq data set for CD4+ T cells isolated from lamina propria of mice infected with different pathogens (Kiner et al. 2021). (d) dot plot graph highlighting the top20 highly expressed genes in the Th2 cells induced by Heligmosomoides polygyrus (Kiner et al., 2021) within each cluster of IL-10 producing CD4+ T cells in the small intestine lamina propria from naïve and Giardia-infected mice.
