Extended Data Fig. 5: Visualisation of HIV-1 WT core nuclear trafficking and uncoating.

a, A representative tomographic slice of a correlatively-acquired tomogram containing WT cores. Two WT cores were identified and indicated by purple arrowheads and numbered: No.1, an imported tube-shaped WT core being transported in the nucleus with discernable surrounding densities; No.2, a docked cone-shaped WT core on the NPC, shown on another slice of the same tomogram (white framed). The zoomed-in view of the HIV-1 WT core en route is depicted in the middle panel. The mapped-back model of this WT core is depicted in the right panel, CA pentamers are highlighted in gold colour. (b-d) Representative tomographic slices of correlatively-acquired tomograms of WT cores uncoating in the nucleus: incipient (b), half-way (c), and nucleic acid-releasing (d). In b, the uncoating point is indicated by the light blue arrowhead. In c, half of the capsid could not be detected by template matching and is apparently missing. In d, nucleic acids can be seen releasing from the remaining capsid shell; nucleic acid density is traced as indicated by light blue arrowheads. Mapped-back models and segmented volumes are illustrated in the right panel. The NPC, ribosomes, and nucleosomes, surrounding nuclear factors, and prominent linker DNA between nucleosomes are labelled. The nucleus, nuclear envelope (NE) and membranes are annotated accordingly. Scale bar = 100 nm.