Extended Data Fig. 9: Characterisation of WT and mutant HIV-1 cores. | Nature Microbiology

Extended Data Fig. 9: Characterisation of WT and mutant HIV-1 cores.

From: HIV-1 nuclear import is selective and depends on both capsid elasticity and nuclear pore adaptability

Extended Data Fig. 9

a, A violin plot of the statistical analysis on the size of cone-shaped HIV-1 cores (width measured at the wide end) from all samples incubated with PS-CEM cells. The size of cone-shaped WT cores measures 56.11 ± 5.053 nm (SE = 0.2193, n = 530), the E45A measures 56.43 ± 2.919 nm (SE = 0.2916, n = 412), the E45A/R132T measures 55.10 ± 7.802 nm (SE = 0.8668, n = 79), and the N74D measures 54.36 ± 5.587 nm (SE = 0.7466, n = 56). White lines represent the medians, black lines represent the quartiles, and black dots represent individual cone-shaped cores (One-way ANOVA test for all, ns = no significance). b, A violin plot of the statistical analysis on the size of tube-shaped cores (width measured) from all samples incubated with PS-CEM cells. The size of tube-shaped WT cores measures 41.38 ± 3.157 nm (SE = 0.2535, n = 155), the E45A measures 42.29 ± 4.071 nm (SE = 0.5170, n = 62), the E45A/R132T measures 40.90 ± 1.758 nm (SE = 0.3836, n = 23), and the N74D measures 41.52 ± 2.871 nm (SE = 0.6962, n = 17). White lines represent the medians, black lines represent the quartiles, and black dots represent individual tube-shaped HIV-1 cores (One-way ANOVA test for all, ns = no significance). c, A line chart illustrating the percentage of cone-shaped HIV-1 cores in each state of all samples incubated with PS-CEM cells. d, A line chart illustrating the percentage of tube-shaped HIV-1 cores in each state of all samples incubated with PS-CEM cells.

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