Extended Data Fig. 10: Characterisation of HIV-1 E45A/R132T core nuclear import. | Nature Microbiology

Extended Data Fig. 10: Characterisation of HIV-1 E45A/R132T core nuclear import.

From: HIV-1 nuclear import is selective and depends on both capsid elasticity and nuclear pore adaptability

Extended Data Fig. 10

a, Gallery of E45A/R132T cores in multiple states during nuclear import. Six representative tomographic slices from each state are shown. E45A/R132T cores are indicated by purple arrowheads, nuclear factors are indicated by light purple arrowheads, the nucleus, NE (indicated by black dashed lines in some cases), and NPC (indicated by white circles in the top view in some cases) are annotated accordingly. Scale bars = 50 nm. b, A bar chart illustrating the composition of E45A/R132T core shapes in each state. Cone-shape cores are in purple and tube-shaped cores in blue (Two-sided Chi-square test for all, p < 0.0001). c, A bar chart showing the orientation distribution of cone-shaped E45A/R132T cores in docking and traversing states, with the wide end in first (grey) and narrow end in first (light purple) (Two-sided Fisher’s exact test for one comparison, p = 0.0022). d, A violin plot of the statistical analysis on the size of cone-shaped E45A/R132T cores (width measured at the wide end) in each state. The size of imported cone-shaped E45A/R132T cores measures 51.57 ± 3.867 nm (SE = 1.462, n = 7), the traversing measures 53.32 ± 6.027 nm (SE = 1.285, n = 22), the docking measures 56.96 ± 8.164 nm (SE = 1.666, n = 24), the approaching measures 56.88 ± 8.454 nm (SE = 1.658, n = 26), and the input measures 58.66 ± 9.166 nm (SE = 0.7560, n = 147). White lines represent the medians, black lines represent the quartiles, and black dots represent individual cone-shaped E45A/R132T cores (One-way ANOVA test for all, ns = no significance). e, A violin plot of the statistical analysis on the size of tube-shaped E45A/R132T cores (width measured) in each state. The size of imported tube-shaped E45A/R132T cores measures 40.92 ± 2.109 nm (SE = 0.6088, n = 12), the traversing measures 41.50 ± 2.121 nm (SE = 1.500, n = 2), the docking measures 41.00 ± 1.000 nm (SE = 0.5774, n = 3), the approaching measures 40.83 ± 1.472 nm (SE = 0.6009, n = 6), and the input measures 42.63 ± 3.460 nm (SE = 0.5688, n = 37). White lines represent the medians, black lines represent the quartiles, and black dots represent individual tube-shaped E45A/R132T cores (One-way ANOVA test for all, ns = no significance).

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