Extended Data Fig. 5: Circular dichroism spectra of archaeasins.
From: Deep learning reveals antibiotics in the archaeal proteome
Circular dichroism experiments were conducted with encrypted peptides from the archaeome using a J-1500 Jasco circular dichroism spectrophotometer. The spectra were recorded in three different media: (a) water, (b) 60% trifluoroethanol in water, and (c) sodium dodecyl sulfate (SDS) in water (10 mmol L−1), after three accumulations at 25 °C, using a 1 mm path length quartz cell, between 260 and 190 nm at 50 nm min−1, with a bandwidth of 0.5 nm. The concentration of all peptides tested was 50 μmol L−1. (d) Heatmap with the percentage of secondary structure found for each peptide in three different solvents: water, 60% trifluoroethanol (TFE) in water, and SDS (10 mmol L−1) in water. Secondary structure fraction was calculated using the BeStSel server18.
