Extended Data Fig. 6: Outer membrane permeabilization and cytoplasmic membrane depolarization of A. baumannii ATCC 19606 induced by archaeasins.
From: Deep learning reveals antibiotics in the archaeal proteome
(a-c) Outer membrane permeabilization was assessed using the probe 1-(N-phenylamino)naphthalene (NPN), showing the permeabilization effects of Archaea-encoded encrypted peptides active against A. baumannii ATCC 19606: (a) relative fluorescence, (b) fluorescence intensity, and (c) summary of the relative fluorescence in different time points of the experiment. (d-f) Membrane depolarization assays were performed using the hydrophobic probe 3,3′-dipropylthiadicarbocyanine iodide [DiSC3-(5)] on all archaeasins active against A. baumannii ATCC 19606: (d) relative fluorescence, (e) fluorescence intensity, and (f) summary of the relative fluorescence in different time points of the experiment. Polymyxin B served as a positive control, while buffer, buffer with the probe, and buffer with both probe and bacteria were used as baseline controls for fluorescence. Error bars are the standard deviation obtained from the three replicates and relative fluorescence is shown as a non-linear regression of the fluorescence intensity normalized by the untreated control (buffer).
