Fig. 3: Synergistic interaction between archaeal peptide antibiotics.
From: Deep learning reveals antibiotics in the archaeal proteome
The synergistic interactions between pairs of EPs from the same or closely related organisms (phylogenetic pairwise distance ≤8) that showed activity against A. baumannii ATCC 19606 were assessed using checkerboard assays. These assays involved twofold serial dilutions, ranging from 2× MIC to a 1:32 dilution. The histogram shows the FICI values obtained for each pair of EPs. A total of 79 pairs were evaluated. Low FICI values (≤0.5) indicate synergistic interactions, intermediate values (0.5 < FICI ≤ 1) indicate additive effects and higher values (1 < FICI ≤ 2) indicate indifferent interactions. Numbers 1–15 indicate where each pair or group of pairs originates within the archaeal phylogenetic tree.
