Fig. 1: VZV modulates the proteome signature of infected neuronal cells.
a, Experimental design of the VZV–host proteomic survey. Neuroblastoma SK-N-BE2 cells were infected with VZV, and the effects of infection on their proteome were analysed by bottom-up MS to generate the infection dataset. SK-N-BE2 cells were transduced with individual V5-tagged VZV ORFs, and analysed by MS after AP of the tagged viral bait (interactome dataset) and on the proteome level (effectome dataset). b, Volcano plot of VZV-induced protein abundance changes in SK-N-BE2 cells infected with VZV for 48 h. Significant host protein changes (two-sided Student’s t-test, permutation-based FDR ≤ 5 × 10−2, |median log2-transformed fold change| ≥ 0.5, n = 4 independent experiments) are marked in dark grey or coloured according to their GO annotation as presented in panel c. Viral proteins are coloured in red. The plot displays one representative assay of two repeats, each including four independent experiments (Supplementary Table 1). The bigger circles highlight changes observed in the two repeats. Diamonds indicate truncated log2-transformed fold change. c, GO terms enriched among the cellular proteins that are downregulated or upregulated in VZV-infected SK-N-BE2 cells as represented in panel b (one-sided Fisher’s exact test, Benjamini–Hochberg FDR ≤ 5 × 10−2, enrichment factor ≥ 4.5). Regulated GO terms were grouped and coloured according to parental cellular functions, as defined in the legend in b.
