Extended Data Fig. 10: het-S/R0Agp confers resistance to T5.
From: Characterization of an amyloid-based antiphage defence system in Escherichia coli
a, Spot assay with 10-fold serial dilutions of T5 on lawns of MG1655 cells and containing a plasmid with het-S/R0Agp and mutant versions of the same construct (het-S Q240P and R0Agp H9P). MG1655 cells and MG1655::Bab/Agp cells carrying the empty vector are used as controls. Each dilution is spotted in triplicates, serial dilutions are from top to bottom and left to right as in Fig. 2b. b, Growth curves of strains used in a. and infected with T5 at different MOIs as indicated (NI, non-infected). Curves are given as means +/− s.d. of three biological replicates (the same bacterial cultures has been infected in triplicates), (bars may not appear on the graph if smaller than symbols). c, Representative fields in microscopic examination of strains expressing HET-S/R0Agp, HET-S Q240P/R0Agp and HET-S/R0Agp H9P infected with T5 at MOI 10 and imaged 45 min after infection (NI, non infected). Cell ghosts are present in HET-S R0Agp cultures while most cells are lysed in the Q240P and H9P mutants. Scale bar is 5 µm.
