Extended Data Fig. 3: Formation of dot-like foci by BASS11Bab-GFP in E. coli.
From: Characterization of an amyloid-based antiphage defence system in Escherichia coli
a, Examples of colocalization of BASS11Bab-GFP foci with refringent inclusion bodies at the cell poles, scale bar is 2 µm. Two examples of representative fields are shown. b, Examples of cells with BASS11Bab-GFP cable-like structures, scale bar is 4 µm. Cables are observed in 3.3%±0.6 of the cells (means +/− s.d. of three technical replicates). c, Representative examples of colonies obtained after plating liquid cultures containing cells with dots and cells with diffuse BASS11Bab-GFP fluorescence: from top to bottom, a colony homogeneously displaying cells with dots, a colony homogeneously displaying cells with diffuse fluorescence and a chimeric sectored colony with a dot and a diffuse fluorescence sector. Colonies were imaged after 6 h of growth at 37° on solid LB medium. Scale bar is 10 µm. d, Correlation between the proportions of dot cells in starting liquid cultures and the proportion of dot colonies formed after plating. The upper graph gives the proportion of cells with dots at four different time points after transformation of MG1655 E. coli cells with a plasmid expressing BASS11Bab-GFP (t1 to t4 are 8h, 12h, 15h and 18h respectively). The two lower graphs give the proportion of dot and chimeric sectored colonies obtained after plating the liquid cultures from the time points t1 to t4. Results are means +/− s.d. of technical replicates (the same culture plated in triplicate). Pearson correlation coefficient between the proportion of cells with dots at t1, t2, t3 and t4 and proportion of colonies with dots at the same time points is r=0.9874 (two tailed p-value=0.0126, CI for r (0.5143; 0.9997)).
