Extended Data Fig. 5: Percent survival of clinically isolated in absence or presence of formate plus micronomicin. | Nature Microbiology

Extended Data Fig. 5: Percent survival of clinically isolated in absence or presence of formate plus micronomicin.

From: Metabolic reprogramming enhances the susceptibility of multidrug- and carbapenem-resistant bacteria to antibiotics

Extended Data Fig. 5

a and b, Percent survival of MDR-ECO (a) and CR-ECO (b) in the presence or absence of 20 mM sodium formate and 60 μg/mL of micronomicin, except for 30 μg/mL for MDR-ECO31, MDR-ECO37, MDR-ECO42, 20 μg/mL for MDR-ECO38, 5 μg/mL for MDR-ECO33, MDR-ECO40, 2 μg/mL for MDR-ECO34, 1 μg/mL for MDR-ECO47, MDR-ECO48, MDR-ECO50, 0.5 μg/mL for MDR-ECO32, 30 μg/mL for CR-ECO1, 20 μg/mL for CR-ECO10, CR-ECO13, 1 μg/mL for CR-ECO5, CR-ECO19, CR-ECO22, and 0.5 μg/mL for CR-ECO12. n = 3. a, ****p < 0.0001 for all comparisons between MCR+for-Na and the other three groups. b, ****p < 0.0001 for all comparisons between MCR+for-Na and the other three groups. c, Percent survival of MDR-K. pneumonia in the presence or absence of 20 mM sodium formate and micronomicin 60 μg/mL for MDR-KPN23 and ATCC700603, 1.5 μg/mL for MDR-KPN4, 1 μg/mL for MDR-KPN42. n = 3. ****p < 0.0001 for comparisons between MCR+for-Na and the other three groups. d, Percent survival of CR- and MDR-P. aeruginosa in the presence or absence of 20 mM sodium formate and micronomicin 40 μg/mL for CR-PAE-A2, 5 μg/mL for CR-PAE-C3, 2 μg/mL for MDR-PAE3 and PAE-S1, and 0.5 μg/mL for CR-PAE-D2. n = 3. ****p < 0.0001 for all comparisons between MCR+for-Na and the other three groups. e, Percent survival of methicillin-resistant and -sensitive Staphylococcus aureus (MRSA and MSSA, respectively) in the presence or absence of 20 mM sodium formate and micronomicin 10 μg/mL for MRSA and 1 μg/mL for MSSA. n = 3. ****p < 0.0001 for all comparisons between MCR+for-Na and the other three groups. f, Percent survival of the indicated antibiotic-resistant pathogens in the presence or absence of 20 mM sodium formate and 5 μg/mL micronomicin for Vibrio paraheamolyticus ZNV4 and ZNV10, and Edwardsiella tarda EIB202. n = 3. ****p < 0.0001 for all comparisons between MCR+for-Na and the other three groups. All error bars represent standard deviation. All statistical tests were two-sided, created in Graphpad Prism 10. Data were performed by two-way ANOVA followed by Sidak’s multiple comparison test.

Source data

Back to article page