Extended Data Fig. 1: Antibiotic selection and effect of the combination of micronomicin and pyruvate on S-ECO, MDR-ECO, and CR-ECO.
a, Percent survival of MDR-ECO44 and CR-ECO28 in the absence or presence of 10 mM sodium pyruvate (Pyr-Na) with the indicated antibiotics. GEN (Gentamicin, 60 μg/mL), AMI (amikacin, 60 μg/mL), MCR (micronomicin, 60 μg/mL), MEM (meropenem, 1 μg/mL), IPM (imipenem, 1 μg/mL), CAZ (ceftazidime, 20 μg/mL), SCF (cefoperazone-sulbactam, 20 μg/mL), BLFX (balofloxacin, 10 μg/mL), and CIP (ciprofloxacin, 10 μg/mL) for 10 h. n = 3. ****p < 0.0001 at GEN, AMI, MCR, CIP; p = 0.0002 at BLFX in MDR-ECO44. b, Percent survival of S-ECO, MDR-ECO, and CR-ECO in the presence or absence of 10 mM sodium pyruvate and plus 20 μg/mL, 60 μg/mL, and 240 μg/mL micronomicin, respectively, for 10 h. b, n = 3. ****p < 0.0001 for all but ***p = 0.0002 (51). All error bars represent standard deviation. All statistical tests were two-sided, created in Graphpad Prism 10. Data were performed by two-way ANOVA followed by Sidak’s multiple comparison test.
