Fig. 2: Dependence of Ume6 binding on Efg1.
a, Heatmaps of the ChIP-seq data showing Ume6 occupancies in SC5314 and P75010 ranked by their binding affinity. Regions ±2 kb around Ume6 peak summits are shown. The average read coverage profiles are shown above the heatmaps. Pearson coefficients between SC5314 and P75010 are 0.83–0.87. Each heatmap represents three biological replicates (n = 3). b, Heatmaps of the ChIP-seq data showing Ume6 occupancies in the wild type, efg1Δ/Δ mutant, wild type overexpressing UME6 and efg1Δ/Δ mutant overexpressing UME6 in the genome. Regions ±2 kb around Ume6 peak summits are shown. The average read coverage profiles are shown above the heatmaps. Pearson coefficients between the wild type and efg1Δ/Δ mutant with overexpressed UME6 strains are 0.71–0.82. Each heatmap represents three biological replicates (n = 3). c,d, Venn diagrams depicting the intersection between Ume6-bound genes and either Efg1-bound genes or Efg1 core targets. The grey and blue circles indicate Efg1-bound genes and Efg1 core targets, respectively. Statistical significance was determined using Fisher’s exact test (significance for Ume6-bound genes and Efg1-bound genes: P < 2.2 × 10−16; significance for Ume6-bound genes and Efg1 core targets: P < 2.2 × 10−16). e, Heatmaps of the ChIP-seq data showing Ume6 occupancies at 62 targets in the wild type, efg1Δ/Δ mutant, wild type overexpressing UME6 and efg1Δ/Δ mutant overexpressing UME6 in the genome. Regions ±2 kb around Ume6 peak summits are shown. The average read coverage profiles are shown above the heatmaps. Each heatmap represents three biological replicates (n = 3). f, Genome browser tracks showing the Ume6 binding sites in the upstream region of hypha-associated genes HWP1, HGC1, ALS3 and ECE1 in each strain. The y axis indicates read counts. The track of the untagged strain was used as a control. The ChIP-seq tracks represent three biological replicates (n = 3). All strains used in this figure are homozygous for alleles indicated. The WT genotype is UME6-HA/UME6-HA. In a, b and e, each colour scale bar indicates the peak intensity, which is calculated as the log2 ratio of signals from the immunoprecipitated sample versus the input sample.
