Extended Data Fig. 7: OAA promotes ETS2 phosphorylation at S313 by TAOK1.
From: Oxaloacetate sensing promotes innate immune antiviral defence against influenza virus infection
a–c, Immunoprecipitation and immunoblot analysis of THP-1 cells treated DEOAA (1 mM) with indicated antibodies. d, Total, cytosolic, and nuclear fractions of THP-1 cells transfected with Scr or TAOK1-specific siRNA in the presence of DEOAA (1 mM) were immunoblotted with indicated antibodies. e, Representative image of HeLa cells transfected with Scr or TAOK1-specific siRNA in the presence of DEOAA (1 mM), followed by labelling of ETS2 (green) with specific antibody. Scale bar, 20 μm. f, Quantitative analysis of the similar samples as (e) from three biologically independent experiments (20 cells scored per condition per experiment). g, Total, cytosolic, and nuclear fractions of 293 T cells transfected with Flag-TAOK1 (WT or K57A) were immunoblotted with indicated antibodies. h, Representative image of HeLa cells transfected with plasmids encoding WT and the indicated mutant form of ETS2, together with TAOK1 (WT or K57A), followed by labelling of ETS2 (green) with specific antibody. Scale bar, 20 μm. i, Quantitative analysis of the similar samples as (h) from three biologically independent experiments (20 cells scored per condition per experiment). j, Total, cytosolic, and nuclear fractions of 293 T cells transfected with HA-tagged WT, K313A or K313D mutant form ETS2 were subjected to immunoblotting. k, 293 T cells transfected plasmids of HA-ETS2 and Flag-tagged WT MDH1 as well as its indicated mutants were treated with DEOAA (1 mM). The extracts of were immunoprecipitated using phosphoserine/theonine/tyrosine polyclonal antibody and immunoblotted with ETS2 antibody. l, Extracts of THP-1 cells transfected with Scr, MDH1- and/or TAOK1-specific siRNAs and overexpressed ETS2 S313D mutant were analyzed by immunoblotting. f,i, Data are represented as mean ± s.e.m. (n = 3 independent biological experiments). P values were determined using two-tailed unpaired Student’s t test. a–e,g,h,j–l, Similar results were obtained in 3 independent biological experiments.
