Extended Data Fig. 2: OAA is involved in antiviral responses.
From: Oxaloacetate sensing promotes innate immune antiviral defence against influenza virus infection
a, THP-1-derived macrophages cultured within 13C4 OAA (5 mM) for 12 h with either PBS or H1N1 (PR8) virus (MOI = 1) treatment for 8 h. Schematic representation of 13C4 OAA-derived carbon tracing of metabolites. b–i, Isotopomer analysis of 13C4 OAA-derived carbon incorporation of N-acetyl-D-glucosamine 6-phosphate (GlcNAc6P) (b), D-fructose 1,6-bisphosphate (Fructose-1,6P2) (c), phosphoenolpyruvate (PEP) (d), pyruvate (e), acetyl-CoA (f), citrate (g), malate (h), and aspartate (i) in THP-1-derived macrophages treated with either PBS or H1N1 (PR8) virus (MOI = 1) for 8 h in addition to 13C4 OAA treatment for 12 h (n = 3). j, qPCR analysis of mRNA level of indicated genes in THP-1 cells expressing corresponding single-guide RNAs (sgRNAs). k, THP-1 cells expressing indicated sgRNA were challenged with H1N1 (PR8) virus (MOI = 1) for 24 h, and the supernatants were collected for plaque assay. b–i, Data are represented as mean ± s.d. j,k, Data are represented as mean ± s.e.m. (n = 3 independent biological experiments). P values were determined using two-tailed unpaired Student’s t test.
