Extended Data Fig. 8: Growth inhibition and in-vivo efficacy of phages against two ECC isolates (A-F).
(A) Growth kinetics (OD600) of ECC isolate APO57 over 24 h following treatment with cocktail-V1 and its phage components. Positive control represents bacterial growth without phage treatment. (B) Growth kinetics (OD600) of APO57 treated with Entelli-02 and its phage components. (C) In-vivo phage titres of different phages recovered from tissues of mice infected with APO57 and treated with Entelli-02. Data points represent individual mice (n = 6/group × 4 organs or tissue). Box plots show median (centre line), interquartile range (box edges: 25th-75th percentiles), whiskers extending to the most extreme values within 1.5× interquartile range, and outliers as individual points beyond whiskers. Statistical analysis was performed using the Mann-Whitney U test (two-sided) with exact p-values to compare medians of phage count between groups. (D) Growth kinetics (OD600) of ECC isolate ALF22D176 challenged with cocktail-V1 and its phage components. (E) Growth kinetics (OD600) of ECC isolate ALF22D176 challenged with Entelli-02 and its phage components. (F) In-vivo phage titres recovered from tissues of mice infected with ALF22D176. Box plots show phage titres in four organs/tissues, with medians, interquartile ranges, and outliers indicated as above. Data points represent individual mice (n = 6/group × 4 organs or tissue). Statistical analysis was performed as above. Dotted lines in panels C and F represent detection limits.
