Fig. 2: Receptors of three phages of cocktail-V1.
a, Schematic representation of the Enterobacter LPS and membrane structure, with validated phage receptors indicated by arrows. Colours are for illustrative purposes only and the faded section represents the potential loss of surface-associated structure in phage-resistant mutants. Figure drawn on the basis of ref. 96 using Inkscape. b, SNPs identified in the genome of øEnA02-resistant mutant (ø-R-mutant) and predicted effect on wcaJ. The graph on the right shows phage adsorption assay testing the ability of phages to adsorb to its corresponding WT, phage-resistant mutant and complemented host over 10 min. c, SNPs identified in the genome of øEnA02-resistant mutant and predicted effect on ompW with phage adsorption assay as shown in the graph on the right. d, SNPs identified in the genome of øEnC07-resistant mutant and predicted effect on GT2 with phage adsorption assays as shown in the graph on the right. e, SNPs identified in the genome of øEnC15-resistant mutant and predicted effect on galU with phage adsorption assay as shown in the graph on the right. WT indicates wild-type host, ø-R-mutant indicates phage-resistant mutant, and numbers represent nucleotide positions for the respective genes. Data represent mean ± s.e.m of 3 biological replicates.
