Fig. 4: Targeted phage isolation and expansion of host range.
a, Host-range map of isolated phages and cocktail-V1 tested against the initial subset of 36 ECC isolates. Each row represents a phage. STs are colour coded. Singletons are STs that occurred once. Red boxes show complete lysis and light blue boxes show no lysis. b, Representations of phage genome size and classification of the phages øNando and øTaquito. c,d, TEM images of the øNando (c) and øTaquito (d) phages. e, Schematic representation of the Enterobacter LPS and membrane structure, with putative phage receptors indicated by arrows. Colours are for illustrative purposes only and the faded section represents the potential loss of LPS structure in phage-resistant mutants. Figure drawn on the basis of ref. 96 using Inkscape. f,g, One-step growth curves of øNando (f) and øTaquito (g), propagated on their host of isolation. Data represent mean ± s.e.m. of 3 biological replicates. The latency time (in minutes) and burst size (in p.f.u.s per infection) were calculated for each phage. h, Stability tracking of øNando and øTaquito over 18 months (M), with storage at 4 °C and room temperature. Data represent mean ± s.e.m. of 2 biological replicates.
