Extended Data Fig. 1: Efficacy of the phage cocktail-V1 against mouse infections and its host coverage across a range of clinical isolates.
(A) Experimental scheme for the animal experiment. Grey numbers represent number of mice, black numbers represent hours post-infection. The illustration was created using Inkscape. (B) The reduction of bacterial load in vital tissues of control mice compared to the treatment group. Each data point represents an individual mouse (n = 6). Box plots show median (centre line), interquartile range (box edges: 25th-75th percentiles), whiskers extending to the most extreme values within 1.5× interquartile range, and outliers as individual points beyond whiskers. Total represents data from all samples (n = 24) combined. Statistical analysis was performed using the Mann-Whitney U test (two-sided) with exact p-values to compare medians of bacterial count between groups. (C) The propagation of three different phages in various tissues of treated mice. The data (n = 6) are shown in box plots as above. Statistical analysis was performed using the Mann-Whitney U test as above. (D) Host range map of cocktail-V1 (øEnA02, øEnC07, and øEnC15) and its individual components tested against 120 Enterobacter cloacae complex (ECC) isolates from The Alfred Hospital. Each column represents an ECC isolate classified based on their Sequence Type (ST) and is color-coded accordingly. Red boxes indicate complete phage lysis of the respective ECC isolate, yellow boxes represent partial lysis, and light blue boxes indicate no observed lysis.
