Fig. 4: Newly described BAM components are important for cell envelope integrity and growth in vitro.
From: Structure of a distinct β-barrel assembly machinery complex in the Bacteroidota
a, Coomassie-stained 4–12% SDS-PAGE gel of BtBamAhis pulldowns from wild-type cells and the bamH deletion strain. b, Volcano plot showing enrichment of proteins in pulldowns from B. thetaiotaomicron bamAhis ΔbamH versus the wild-type strain (n = 3 biological replicates for both conditions). The vertical dashed lines represent log2(fold change) = ±1. Proteins above the horizontal dashed line have an adjusted P < 0.05. Statistical significance was inferred by two-sided t-test with Benjamini–Hochberg correction for multiple comparisons. c, Growth of wild-type and ΔbamH B. thetaiotaomicron strains in EBHI (top), minimal medium with 0.5% fructose (MM-Frc; middle) and minimal medium with 0.4% dextran 40 and 10% enriched brain–heart infusion (MM-Dex; bottom). Each trace is an average of n = 3 technical repeats; the error bars represent the s.d. d, Growth of wild-type and ΔbamH B. thetaiotaomicron strains in EBHI in the presence of indicated concentrations of SDS and sodium deoxycholate. Each trace is an average of n = 3 technical repeats; the error bars represent the s.d. Each experiment is representative of 2 or 3 biological replicates. e, Growth of P. gingivalis wild type, ΔbamH, ΔbamI and ΔbamJ in rich medium (tryptic soy broth/yeast extract, TSBY) and minimal medium (DMEM) supplemented with BSA and regular or low amounts of vitamin K (0.5 mg l−1 and 0.05 mg ml−1, respectively) and haemin (5 mg ml−1 and 0.5 mg ml−1, respectively). Each data point is the average of n = 3 biological repeats; the error bars represent the s.d.
