Extended Data Fig. 4: Tolerance to PmB is conserved under nutrient-limiting conditions.

a. Survival of stationary phase Pseudomonas aeruginosa PA14 exposed to 4 µg ml⁻¹ PmB in MM ± glucose, as determined by CFU counts. b, Survival of a panel of E. coli clinical isolates cultured to stationary phase, exposed to 4 µg ml⁻¹ PmB in MM ± glucose for 2 h. c, Survival of C. freundii and E. asburiase clinical isolates cultured to stationary phase, exposed to 4 µg ml⁻¹ PmB in MM ± glucose for 2 h. d, Survival of a K. pneumoniae clinical isolates cultured to stationary phase, exposed to 4 µg ml⁻¹ PmB in MM ± glucose for 2 h. e, Survival of P. aeruginosa clinical isolates cultured to stationary phase, exposed to 4 µg ml⁻¹ PmB in MM ± glucose for 2 h. f, Survival of A. baumannii clinical isolates cultured to stationary phase, exposed to 4 µg ml⁻¹ PmB. g, OM disruption of stationary phase P. aeruginosa cells during the first 20 min of exposure to 4 µg ml⁻¹ PmB, as determined by uptake of the NPN fluorescent dye. h, OM and IM disruption of stationary phase P. aeruginosa exposed to 4 µg ml⁻¹ PmB in MM ± glucose, as determined by uptake of the fluorescent dye SYTOX green. For g, and h, the blank value refers to the relevant fluorophore in medium without bacteria. All experiments were replicated in n = 3 independent assays, error bars show the standard deviation of the mean. Significant differences were determined by two-way (a - h) repeated measures ANOVA. P= *<0.05, **<0.01, ***<0.001, ****<0.0001, ns=not significant.

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