Extended Data Fig. 7: PmB killing requires ATP.
From: Polymyxin B lethality requires energy-dependent outer membrane disruption
a, Survival of stationary phase E. coli exposed to 4 µg ml−1 PmB in MM ± equimolar concentrations of different sugars, as determined by CFU counts. b, Standard curve plotting Log10 ATP concentration (nM) against Log10 luminescence counts. Simple linear regression was performed using prism version 10.4.1, where the blue line represents the line of best fit, and the red dotted line the 95% confidence interval. c, ATP concentration according to standard curve interpolation, of stationary phase E. coli incubated in equimolar concentrations of different sugars across a 30-minute time course. d, Four-way correlation matrix showing correlation coefficients of PmB susceptibility, ATP concentration and densitometric analysis pre- and post-PmB treatment. All experiments were replicated in n = 3 independent assays. Error bars show the standard deviation of the mean.
