Extended Data Fig. 8: PmB killing requires LPS synthesis.
From: Polymyxin B lethality requires energy-dependent outer membrane disruption
a, b, c Survival of stationary phase P. aeruginosa exposed, or not, to 4 µg ml−1 PmB in MM + G with or without 1X MIC of LpxC inhibitors CHIR-090 (a) or ACHN-975 (b) or the protein synthesis inhibitor tetracycline (c). (d) AFM phase images showing stationary phase E. coli MG1655 cells exposed to 2.5 µg ml−1 PmB in the presence or not of 0.125 µg ml−1 CHIR-090 in MM + G, shown as a function of time. (e) AFM phase images showing stationary phase E. coli imp4231 cells exposed to 2.5 µg ml−1 PmB in the presence or not of 0.5 µg ml−1 G907 in MM + G, shown as a function of time. Scalebar: d, e 250 nm. Phase scale (scale inset in first row of d and e, at t = 90 min): d, 2.5 deg, e 4 deg (top), 6 deg (bottom) All experiments were replicated in n = 3 independent assays. Error bars show the standard deviation of the mean. Significant differences were determined by two-way repeated measures ANOVA between PmB-treated and PmB + antibiotic-treated conditions. P= **<0.01, ns=not significant.
