Extended Data Fig. 1: Polymyxin B lethality requires metabolic activity.
From: Polymyxin B lethality requires energy-dependent outer membrane disruption
a, Visual representation of the set-up for all microbiological assays. E. coli MG1655 was grown in 3 ml of MHB for 16 h at 37oC 180 r.p.m. to stationary phase. The culture was washed three times in MM and resuspended at an inoculum density of 1×108 in 3 ml of MM or MM + G. To test exponential phase E. coli, the overnight culture was diluted 1/1000 in fresh MHB and grown for a further 3 h. b, Survival of stationary phase E. coli exposed to 4 µg ml−1 PmB from t = 0 in the presence of a 1/10 dilution series of 0.36% glucose. A 1/100 dilution of 0.36% glucose supported PmB killing, however, a 1/1000 dilution substantially reduced the rate and degree of PmB killing. c, Survival of stationary phase E. coli exposed to 4 µg ml−1 PmB from t = 0 in MM + G or MM with an equimolar concentration of 2-deoxy-D-glucose, as determined by CFU counts. All experiments were replicated in n = 3 independent assays. Error bars show the standard deviation of the mean. Significant differences were determined by two-way repeated measures ANOVA. P= *<0.05, ***<0.001.
