Extended Data Fig. 8: IFIT2-IFIT3 inhibits replication of the murine Sendai Virus, related to Fig. 6.
(a) HEK293T cells were transfected with GFP expression plasmids containing the indicated 5’ UTRs from Sendai virus (SeV) in the absence or presence of co-transfected IFIT2-IFIT3. Experiments were performed as in Fig. 5e (n = 4 biological replicates). (b) Inducible Flp-In T-REx HEK293 cells expressing no IFIT (black circles), IFIT2 (blue squares), IFIT3 (yellow triangles), or IFIT2 and IFIT3 together (green triangles) were mock-treated or treated with doxycycline (500 ngμl−1) for 24 h and then infected with SeV-F1R-EGFP (0.1 MOI). Supernatant was collected at 42 hpi for titration (n = 3 biological replicates). Statistical analyses: Data are represented as mean ± SEM. (a, b) Ordinary two-way ANOVA with Šídák’s multiple comparisons test and a single pooled variance. **** = p < 0.0001, ns = not significant.
