Extended Data Fig. 5: Testosterone and DHT activate quorum sensing in S. aureus without affecting growth, and other hormone classes have no effect on quorum sensing.
From: Skin androgens regulate Staphylococcus aureus pathogenicity via quorum sensing
a, Growth curve of HG003 (agr Type I) treated with 10 nM testosterone or DHT. ns by two-way ANOVA compared to Vehicle. b, Bioluminescence of agr-P3 reporter (HG003agrP3::lux) treated with testosterone, DHT, progesterone, or estradiol. #p < 0.0001 or ns by two-way ANOVA com-pared to estradiol. c, qRT-PCR of S. aureus HG003 strain (agr Type I) treated with 10 nM testos-terone or AIP-I (n = 3) till early-growth phase (OD600 0.6). Expression of target genes RNAIII and agrA normalized to gyrA expression. **p < 0.001, *p < 0.05by two-tailed unpaired t-test. d, Growth curve of USA100 (agr Type II) treated with 10 nM testosterone or DHT. ns, not significant by two-way ANOVA compared to vehicle. e, In vitro bioluminescence of USA100 (USA100 agrP3::lux) treated with 10 nM of testosterone or DHT (n = 3). #p < 0.0001, by two-way ANOVA compared to vehicle control. f, qRT-PCR of USA100 treated with 10 nM testosterone or respective AIP (n = 3) through mid-log phase (OD600 0.6). Expression of target genes RNAIII normalized to gyrA expres-sion. Means ± SEM (error bars) are plotted. *p < 0.05by two-tailed unpaired t-test. g, Growth curve of MW2 (agr Type III) treated with 10 nM testosterone or DHT. ns,not significant by two-way ANOVA compared to Vehicle. h, In vitro bioluminescence of MW2 (MW2 agrP3::lux) treated with 10 nM of testosterone or DHT (n = 3). #p < 0.0001, by two-way ANOVA compared to vehicle control. i, qRT-PCR of MW2 treated with 10 nM testosterone or respective AIP (n = 3) through mid-log phase (OD600 0.6). Expression of target genes RNAIII normalized to gyrA expression. Means ± SEM (error bars) are plotted. *p > 0.05, ns, not significant by two-tailed unpaired t-test.
