Extended Data Fig. 1: Greater bacterial growth and skin damage in male mice compared to female mice.
From: Skin androgens regulate Staphylococcus aureus pathogenicity via quorum sensing
a,b, Male and female C57BL/6 wild-type (WT) mice were epicutaneously infected for 4 days with 1×106 colony forming units (CFUs) of a bioluminescent strain of MRSA SAP430 (MRSA::lux). Bioluminescence quantified over time. In vivo bioluminescence images (a) and diseases scores (b) quantified on Day 4. c, Transepidermal water loss (TEWL), a measure of skin barrier damage, quantified by Vapometer on Day 0 and Day 4. d,e, Testosterone (d) and dihydrotestosterone (DHT) (e) quantified from the skin secretions of male and female mice by hormone immunoassay. Means ± SEM are plotted (n = 5/ group). (b) *p < 0.05 by two-sided Kolmogorov-Smirnov Test. (c) **p < 0.001 by two-way ANOVA (two-sided) with Sidak’s correction for multiple comparisons. (d,e) *p < 0.05 **p < 0.01 by Mann-Whitney U-test (two-sided).
